About
Spatter is a software package which performs DNA microarray image analysis. It automatically quantitates two-color microarray images. It performs gridding, segmentation, and data extraction in an efficient and fully-automated manner, and does not require any user interaction while it runs.
Spatter is written in C++ and Python. It makes use of LTI-Lib and the Python Imaging Library. It is developed and tested on Linux, but it may be possible to compile it on other platforms, such as Windows.
The latest version of spatter is 0.5.3, released on 2005-02-25.
License
Spatter is free software, and is distributed under the terms of the GNU General Public License.
Screenshots
Since spatter is a set of command-line tools, there is not much to be seen in the way of screenshots. However, it can produce images for verifying the correctness of grid placement and segmentation results.
Combined two-channel image
Grid overlay
Spot segmentation overlay
Sample Images
The following tarballs each contain sample images, a geometry file for the images, and a makefile for generating gridding and segmentation results using spatter. Note: the tarballs are quite large.
- UHN H19K images (299M)
- UHN M15K images (178M)
- Camda 2004 images (361M)